*After 17 years of marriage and recent separation, George Lopez’s wife Ann has filed for divorce in Los Angeles.

Surrounding the couple’s separation in September, a representative told People.com, “George and Ann Lopez have made the decision to end their 17-year marriage. The decision is amicable and by mutual agreement. They remain dedicated parents, and committed partners in business and their philanthropic organization, The Lopez Foundation.”

Rumors of infidelity began surfacing in May. According to the divorce petition, filed Tuesday in L.A. County Superior Court by Laura Wasser, Ann cites “irreconcilable differences” and asks for spousal support and primary physical custody of their 14-year-old daughter.

According to TMZ, there is no prenuptial agreement.

In 2005, the couple underwent dual operations after Ann gave her husband one of her kidneys. Lopez had a genetic condition that caused his kidneys to deteriorate.